Journal: Scientific Reports

Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice

doi: 10.1038/s41598-025-32446-4

Figure Lengend Snippet: Characterization of immunophenotype of endotheliocytes of sinusoidal capillaries of regenerating liver. ( A ) Dynamics of the population of VCAM-1 + cells in the regenerating liver. ( B ) Dynamics of the population of VE-cadherin + cells in the regenerating liver. Data are presented as mean ± SD, SO – sham splenectomy + sham hepatectomy (n = 6), SE—splenectomy, (n = 6), SH – splenectomy + hepatectomy (n = 18), PH—sham splenectomy + hepatectomy, *—statistically significant differences, p < 0,05. Paired comparisons used the Student’s t-test for normal distributions and the Mann–Whitney U-test for distributions other than normal.

Article Snippet: A 10 5 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130–111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130–122-072, clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130–116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130–128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130–117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130–102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130–102-422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 h. The dynamics of CD3 + lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 10 5 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130–102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APC-labeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBlue-labeled, Biolegend, USA); the incubations proceeded for 1 h. Following the incubations, the cells were washed in PBS, resuspended in 0.5 ml PBS and analyzed in a MACSQuant 10 flow cytometer (Milteniy Biotech, Germany); the data were analyzed in FlowJo (LLC).

Techniques: MANN-WHITNEY